Introduction of Challen GOX (Glucose Oxidase)

Glucose oxidase catalyzes the reaction of glucose with oxygen to form gluconic acid and hydrogen peroxide, which has a significant antibacterial and bactericidal effect. It is an ideal subsitution of some antibiotics. Beijing Challenge Group and Animal Nutrition and Feed Science Institute of Chinese Academy of Agricultural Sciences have jointly overcome several technical difficulties and developed a brand-new Challen GOX with stronger temperature resistance and better efficacy in animal intestinal environment. Challenge Group is the first enterprise to promote glucose oxidase in feed and breeding industry. By extensive trials, the product is testified to effectively control bacterial diarrhea in animals, to reduce the harm of feed mycotoxins to animal organisms, and improve animal performance. Challen GOX have been used widely in China, North America, Europe and Southeast Asia etc.

Chemical and Physical Characteristics

Enzyme Activity: 5000U/g，10000U/g

Appearance: Light yellow power or granule

Package: 25kg/bag

Shelf Life and Storage: 12 months under proper conditions in a dry, well ventilated and cool place with packaging unopened, away from sunlight.

Mode of Action

Glucose oxidase can consume molecular oxygen or atomic oxygen, oxidize glucose, and protect oxidizable components in feed. According to the reaction conditions, there are three forms of Glucose oxidase catalytic reaction :

1 mol of oxygen is consumed when oxidize per 1 mol glucose in the absence of catalase:

C6H12O6+O2→C6H12O7+H2O2；β-D-Glucose+ O2→β-D-Glucose lactone+ H2O2；

1/2 mol of oxygen is consumed when oxidize per 1 mol of glucose in the presence of catalase:

C6H12O6+ 1/2O2→C6H12O7+H2O2；

In the presence of ethanol and catalase, catalase can be used for the oxidation of ethanol. 1 mol of oxygen is consumed when oxidize per 1 mol glucose:

C6H12O6+ C2H5OH+O2→C6H12O7+ CH3CHO +H2O2.

Functions

Reduce the use of antibiotics

Due to the significant antibacterial and bactericidal action of glucose oxidase, Challen GOX can reduce the amount of antibiotics in the feed and make a green feed.

Improve animal intestinal health and reduce intestinal problems

Glucose oxidase can inhibit the growth and reproduction of harmful bacteria in the intestinal tract of animals by consuming oxygen, producing gluconic acid and strong oxidizing substance hydrogen peroxide, improving the intestinal health of the animal, thereby reducing the problems of diarrhea, overfeeding and enteritis problems.

Reduce the risk of mycotoxins to animal organisms

Studies have shown that glucose oxidase can enhance the animal's liver redox reaction in two ways to reduce the harm of mycotoxins to the animal body, and has a significant effect on shortening the sow estrus cycle and improving the reproductive health of poultry.

Improve animal performance

Practice has proved that glucose oxidase can improve feed intake of weaned piglets, reduce piglet diarrhea, reduce the F/G ratio of poultry, increase the egg production rate of layer, and extend egg production peak maintenance time, which has a significant effect on improving animal performance.

Examples of High enzymatic hydrolysis efficiency

Effect contrast of different GOX on corn strarch

Substrate: corn starch 5 g, deionized water 100 mL, 40℃, 15 min；adding 3 drops Methyl red indicator（The stronger the acidity is, the deeper the red is.）

The left breaker: Control; The middle one: adding Challen GOX 0.5 g; The right one: adding one  competitor 0.5g.

Conclusion: Challen GOX acts on starch efficiently , while the competitor doesn’t.

Effect of glucose oxidase on pH of corn-soybean meal dietary

Substrate: (corn: soybean meal = 3:1，mesh 60) 5.0 g + Water 100 mL +1g GOX；40℃，150R/min.

Benefits in Piglets

Two hundred 28-day-old piglets were randomly divided into 2 treatments, each treatment 10 repeats, each repeat 10 piglets. The test period was 15d. The control group added 24 mg/kg flavomycin + 75 mg/kg chlortetracycline, and the treatment added 200g/t Challen GOX HT®+a half antibiotics.

Compared with the control group, the treatment with Challen GOX HT® achieved a better animal  performance. And the feed intake was increased by 86g, the daily gain was increased by 110g, the F/G was reduced by 0.13, and the diarrhea rate was reduced by 7%.

Benefits in Broilers

Four hundred and thirty-two broilers were randomly divided into 2 treatments, each treatment 12 repeats, each repeat 18 broilers. The test period is 42d. Antibiotic control group added 15mg/kg virginiamycin, and the treatment added 200mg/kg GOX HT® .

Compared with the control group with virginiamycin, the treatment with Challen GOX achieved a same animal performance. Although the difference in F/G was not significant, the final body weight was 33.6 g more than the control group.